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fluorescent isothiocyanate fitc labeled concanavalin a  (Vector Laboratories)


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    Vector Laboratories fluorescent isothiocyanate fitc labeled concanavalin a
    Fluorescent Isothiocyanate Fitc Labeled Concanavalin A, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+isothiocyanate+fitc/Fluorescein+Labeled+Concanavalin+A+(Con+A)/bio_rxiv__2025__10__09__681484-195-8-22
    Average 93 stars, based on 142 article reviews
    fluorescent isothiocyanate fitc labeled concanavalin a - by Bioz Stars, 2026-09
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    Incubation:

    Article Title: Nociceptive Afferents to the Premotor Neurons That Send Axons Simultaneously to the Facial and Hypoglossal Motoneurons by Means of Axon Collaterals
    Article Snippet: .. The sections were incubated in 0.5% Triton X-100 in 0.05 M PBS (pH 7.6) overnight prior to incubation in the fluorescent isothiocyanate (FITC) -labeled avidin D (1∶200, A-2001, Vector Laboratories, Burlingame, CA, USA) at room temperature for 2 h. After the incubation, all the sections were rinsed in 0.05 M PBS, mounted onto gelatin-coated glass slides, air-dried, cover-slipped by a mixture of 50% (v/v) glycerin and 2.5% (w/v) triethylene diamine (anti-fading agent) in 0.05 M PBS. ..

    Article Title: Neurochemical Properties of the Synapses in the Pathways of Orofacial Nociceptive Reflexes
    Article Snippet: .. Briefly, the sections were incubated in 0.5% Triton X-100 in 0.05 M PBS (pH 7.6) overnight prior to incubation in the fluorescent isothiocyanate (FITC)-labeled avidin D (1∶200, catalog number: A-2001, Vector Laboratories, Burlingame, CA, U.S.A) at room temperature for 2 h. After the incubation, all sections were rinsed in 0.05 M PBS, mounted onto gelatin-coated glass slides, air-dried, cover-slipped with a mixture of 50% (v/v) glycerin and 2.5% (w/v) triethylenediamine (anti-fading agent) in 0.05 M PBS. ..

    Labeling:

    Article Title: Nociceptive Afferents to the Premotor Neurons That Send Axons Simultaneously to the Facial and Hypoglossal Motoneurons by Means of Axon Collaterals
    Article Snippet: .. The sections were incubated in 0.5% Triton X-100 in 0.05 M PBS (pH 7.6) overnight prior to incubation in the fluorescent isothiocyanate (FITC) -labeled avidin D (1∶200, A-2001, Vector Laboratories, Burlingame, CA, USA) at room temperature for 2 h. After the incubation, all the sections were rinsed in 0.05 M PBS, mounted onto gelatin-coated glass slides, air-dried, cover-slipped by a mixture of 50% (v/v) glycerin and 2.5% (w/v) triethylene diamine (anti-fading agent) in 0.05 M PBS. ..

    Avidin-Biotin Assay:

    Article Title: Nociceptive Afferents to the Premotor Neurons That Send Axons Simultaneously to the Facial and Hypoglossal Motoneurons by Means of Axon Collaterals
    Article Snippet: .. The sections were incubated in 0.5% Triton X-100 in 0.05 M PBS (pH 7.6) overnight prior to incubation in the fluorescent isothiocyanate (FITC) -labeled avidin D (1∶200, A-2001, Vector Laboratories, Burlingame, CA, USA) at room temperature for 2 h. After the incubation, all the sections were rinsed in 0.05 M PBS, mounted onto gelatin-coated glass slides, air-dried, cover-slipped by a mixture of 50% (v/v) glycerin and 2.5% (w/v) triethylene diamine (anti-fading agent) in 0.05 M PBS. ..

    Article Title: Neurochemical Properties of the Synapses in the Pathways of Orofacial Nociceptive Reflexes
    Article Snippet: .. Briefly, the sections were incubated in 0.5% Triton X-100 in 0.05 M PBS (pH 7.6) overnight prior to incubation in the fluorescent isothiocyanate (FITC)-labeled avidin D (1∶200, catalog number: A-2001, Vector Laboratories, Burlingame, CA, U.S.A) at room temperature for 2 h. After the incubation, all sections were rinsed in 0.05 M PBS, mounted onto gelatin-coated glass slides, air-dried, cover-slipped with a mixture of 50% (v/v) glycerin and 2.5% (w/v) triethylenediamine (anti-fading agent) in 0.05 M PBS. ..



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    Image Search Results


    NFIC inhibited the nuclear entry of IRF3(5D ) . 293 T cells were co-transfected with NFIC expression plasmid, IRF3(5D) plasmid or control plasmid (EV) using Lipofectamine 2000 transfection reagent. After 30 h, the cells were fixed with 4% paraformaldehyde and treated with 0.1% Triton X-100. The cells were then incubated with Flag antibody, pIRF3 antibody, Alexa Fluor® 647 fluorescent anti-rabbit secondary antibody or FITC fluorescent anti-mouse secondary antibody. Finally, after staining with DAPI, the cells were observed using a ZEISS laser confocal microscope. Scale bar represents 10 μm. ( B ) Count the number of IRF3(5D)-positive cells and nuclear-translocated cells in 5 random fields, respectively, and calculate the ratio of nuclear-translocated IRF3(5D) cells to total IRF3(5D)-positive cells. ( C ) MARC-145 cells were co-transfected with NFIC expression plasmid, IRF3(5D) plasmid or control plasmid (EV) using Lipofectamine 2000 transfection reagent. After 30 h, nuclear-cytoplasmic fractionation was performed to analyze the protein expression levels of IRF3(5D), Lamin B, GAPDH, Flag-NFIC. To ensure the pIRF3 antibody specifically recognizes IRF3(5D), the

    Journal: BMC Veterinary Research

    Article Title: Nuclear factor IC promoting porcine reproductive and respiratory syndrome virus (PRRSV) replication and suppressing type I interferon transcription

    doi: 10.1186/s12917-025-05230-9

    Figure Lengend Snippet: NFIC inhibited the nuclear entry of IRF3(5D ) . 293 T cells were co-transfected with NFIC expression plasmid, IRF3(5D) plasmid or control plasmid (EV) using Lipofectamine 2000 transfection reagent. After 30 h, the cells were fixed with 4% paraformaldehyde and treated with 0.1% Triton X-100. The cells were then incubated with Flag antibody, pIRF3 antibody, Alexa Fluor® 647 fluorescent anti-rabbit secondary antibody or FITC fluorescent anti-mouse secondary antibody. Finally, after staining with DAPI, the cells were observed using a ZEISS laser confocal microscope. Scale bar represents 10 μm. ( B ) Count the number of IRF3(5D)-positive cells and nuclear-translocated cells in 5 random fields, respectively, and calculate the ratio of nuclear-translocated IRF3(5D) cells to total IRF3(5D)-positive cells. ( C ) MARC-145 cells were co-transfected with NFIC expression plasmid, IRF3(5D) plasmid or control plasmid (EV) using Lipofectamine 2000 transfection reagent. After 30 h, nuclear-cytoplasmic fractionation was performed to analyze the protein expression levels of IRF3(5D), Lamin B, GAPDH, Flag-NFIC. To ensure the pIRF3 antibody specifically recognizes IRF3(5D), the "IRF3" group was transfected with control plasmid and IRF3 expression plasmid simultaneously, serving as a negative control for pIRF3 antibody detection. ( D ) The grayscale values of protein bands in Fig. 6 C were analyzed by using ImageJ, with Lamin B or GAPDH as the internal reference, to compare the fold changes of nuclear-translocated IRF3(5D) in the Flag-NFIC-overexpression group to the control group. Briefly, nuclear IRF3(5D) levels were normalized to Lamin B (value 1), and total IRF3(5D) levels were normalized to GAPDH (value 2). The nuclear translocation ratio of IRF3(5D) was calculated as value 1/value 2, and fold change was determined by comparing the Flag-NFIC-overexpression group to the control group. All experiments were repeated at least three times with similar results. ***, P < 0.01

    Article Snippet: Fluorescein Isothiocyanate (FITC) fluorescent secondary antibody, Alexa Fluor® 647 fluorescent secondary antibody and 4',6-diamidino-2-phenylindole (DAPI) were purchased from Thermo Fisher Scientific.

    Techniques: Transfection, Expressing, Plasmid Preparation, Control, Incubation, Staining, Microscopy, Fractionation, Negative Control, Over Expression, Translocation Assay